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Single chain antibodies (VHH)

Single Chain Antibody Services
Genus Biotech offers a series of services related to single chain antibodies, also known as single domain antibodies, with the best quality and most competitive price.

What is Single Domain Antibody?
Single domain antibodies, also known as domain antibody, VHH, VNAR or sdAb, is a kind of antibody fragments consisting of a single monomeric variable antibody domain lacking the light chain and the CH domain of the heavy chain in conventional Fab region. The first single domain antibody was engineered from the VHH domain of an heavy-chain antibody identified in camelids (e.g. dromedaries, camels, llamas, and alpacas). And the VNAR domain of cartilaginous fishes (e.g. shark) heavy-chain antibody (known as IgNAR, immunoglobulin new antigen receptor) is another qualified source for sdAb development. An alternative approach is to split the dimeric variable domains from normal IgG of humans or mice into monomers by camelizing a few critical residues.

Similar to a conventional antibody, a single domain antibody is able to bind selectively to a specific antigen. In terms of only 12-15 kDa molecular weight, which is much smaller than either full length antibody (150-160 kDa) or other antibody fragments (Fab ~50 kDa, scFv ~25 kDa), single domain antibody takes great advantages of stability and penetrability, which are essential to the development of several antibody drugs or diagnostic tools.

Advantages of Single Domain Antibody

* High stability to function and exist within extreme conditions
* Outstanding penetrability which is able to cross the blood-brain barrier
* Recognize novel epitopes that regular size antibodies cannot
* Improve bioavailability for pharmaceutical applications
* Great potential in downstream engineering (bispecific antibody, humanization)
* Can be expressed in both eukaryotic and prokaryotic systems

Based on our advanced phage display technology, our scientists are specialized in the production and discovery of specific single domain antibodies. According to our client’s demands, we can immunize camel, llama or alpaca as repertoire source to develop single domain antibodies against targets of interest (antigens). Our advanced technology allows various targets to be employed in immunization, including large protein molecules, membrane proteins, haptens and other challenging targets.

Featured sdAb Related Services
* Single Domain Antibody Library Construction and Screening
* Intrabody Discovery
* Premade Single Domain Antibody Library Screening
* Bispecific Single Domain Antibody Production
* Anti-membrane protein sdAb Production
* Single Domain Antibody Humanization
* Single Domain Antibody Conjugation Services
* sdAb Affinity Maturation
* Single Domain Antibody Stability Improvement Service

According to the variety of unique features, single domain antibodies can be used as potential tools for academic research, diseases diagnosis and treatment, and biotechnology development. Genus Biotech is pleased to share our extensive expertise in single domain antibody to facilitate our clients’ research and project development. We can offer high-quality customized services by adjusting protocols to meet even the most specific requirements.

For more information:
tel +39.0824.364090
fax +39.0824.364092

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Genus Biotech
Consorzio Sannio Tech
Via Appia s.n.c. 82030 Apollosa (BN)

phone +39.0824.364090
fax +39.0824.364092

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